Enhancing the security of biological select agents and toxins (BSAT) is a key factor in maintaining a regulatory-compliant select agent program. Currently, there is no technology that can immediately detect concealed BSAT in shipping containers. In an effort to improve the physical security surveillance of BSAT, the U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID) has explored the potential application of RFID technology to detect RFID-labeled biological specimens concealed within shipping containers, without having personnel open the packages. The preliminary findings reveal the differences in the ability of UHF near-field RFID reader antennas and/or a fixed portal reader system to detect various types of RFID tags. The speaker will also discuss the potential applications of RFID to enhance biosecurity capability and the challenges involved in conducting this proof-of-concept.