Ostner, Gregory - St. John's University
Co-authors: Megehee, Elise - St. John's University, Ostner, Gregory - St. John's University, Piotrowski, Cody - St. John's University, Wang, Enju - St. John's University, Wong, Stacey - St. John's University
DNA detection is important to many biological processes. DNA biosensors are increasingly used in hybridization reactions, mutation detection, genomic sequencing, and identification of pathogens. The macromolecular polysaccharide-based polyanions, including heparin salts and carrageenan have unique properties and functions in physiology, and food technology. The quantity of polyanion reflecting the exact number of charges in samples administered in biological procedures has to be strictly controlled. Thus the detection of these polyanions in clinical or commercial samples is key in the diagnostic and quality control processes of related fields.
Novel Os(II) carbonyl complexes with two phenanthroline and a 4-phenyl pyridine, imidazole or phenyl imidazole group exhibit moderate emission intensity in the visible region. Our recent results show that the luminescence intensity of these new osmium complexes can be significantly reduced or enhanced by different DNA strands. This work presents the UV/vis absorbance and luminescence responses of the Os(II) complexes to various DNA samples from natural sources and synthetic; as well as other polyanions The binding constants are calculated with the luminescence and absorbance responses. These studied will provide information in the effort of developing DNA detection markers and/or binding agents for biological applications.
Sensing of oxyanions such as phosphates and carbolylates are very important because of their vital roles in essential biological processes. Our work also studied the responses of Osmium(II) complexes to small anions such as dihydrogen phosphate and acetate anions and the results will be presented.
Application: Bioanalytical
Methodology: Sensors